L5021

Sigma

 

Concanavalin A from Canavalia ensiformis (Jack bean)

gold conjugate, 10 nm nominal (colloidal gold), aqueous glycerol suspension

Synonym:Con A
CAS Number:11028-71-0
MDL number:MFCD00071069

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Description

Analysis NoteWhere reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Biochem/physiol ActionsCon A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Preparation NoteCon A (C7275) adsorbed to colloidal gold; mean particle size 8-12 nm; monodisperse.
Physical formSuspension in 0.02 M Tris buffered saline, pH 8.0, containing 20% glycerol, 1% PEG and 0.05% sodium azide

Properties

formaqueous glycerol suspension
concentration~5 A520 units/mL
total impurities carbohydrate, essentially free
nominal10 nm (colloidal gold)
conjugategold conjugate
storage temp.2-8°C

Safety

WGK Germany3

References

referenceBenhamou, N. and Ouellette, G.B. J. Histochem. Cytochem. 34, 855, (1986) Abstract
 Reeke, G.N., et al. Ann. N.Y. Acad. Sci. 234, 369, (1974)
 Kalb, A.J. and Lustig, A. Biochim. Biophys. Acta 168, 366, (1968)
 Gunther, G.R., et al. Proc. Natl. Acad. Sci. U. S. A. 70, 1012, (1973)
 , Bittiges, H. and Schnebli, H.P, ed. Concanavalin A as a Tool London , (1976)
MerckMerck 13,2521